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A bacterial strain producing a novel endo-inulinase, hydrolysing inulin into oligosaccharides was isolated from soil and identified as Arthrobacter sp. S37. The enzyme production was induced by inulin and Jerusalem artichoke extract. The maximum enzyme production was obtained with medium containing 1.57 jerusalem artichoke extract. 1.0% yeast extract, 0.5% NaNO₃, 0.05% MgSO₄·7H₂O, 0.05% KCl, 0.0016% FeCl₃·6H₂O and 0.05% KH₂PO₄. The optimum temperature and pH for the enzyme production were 30℃ and 8.0, respectively. Under the optimum condition, the enzyme activity in the culture broth reached at maximum. 10.8 units/㎖ after cultivation for 24 hours.
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